Horse Serum, Heat Inactivated – 100 mL is a vital component used extensively in cell culture, immunoassays, and biopharmaceutical research. This serum, derived from equine blood and subjected to heat inactivation, ensures the elimination of complement proteins that may interfere with various laboratory processes. This article provides a detailed overview of its composition, applications, regulatory considerations, and scientific advancements.
Composition and Properties
Horse Serum, Heat Inactivated – 100 mL is obtained from healthy equine donors and processed to retain essential growth factors while eliminating unwanted components. Key features include:
- Heat Inactivation: The serum is heated at 56°C for 30 minutes to deactivate complement proteins.
- Rich in Growth Factors: Provides essential nutrients and hormones for cell growth.
- Sterile and Filtered: Passed through a 0.2-micron filter to remove microbial contaminants.
For further information on serum composition and processing, visit the U.S. National Library of Medicine (NLM).
Clinical and Research Applications
Horse Serum, Heat Inactivated – 100 mL is widely utilized in biomedical and laboratory research. Its primary applications include:
1. Cell Culture and Tissue Engineering
Horse serum provides an essential supplement for cell culture media, promoting the growth of various cell lines, including:
- Fibroblasts: Used in wound healing and regenerative medicine studies.
- Hybridoma Cells: Critical for monoclonal antibody production.
- Stem Cells: Supports proliferation in tissue engineering applications.
For more insights, refer to American Type Culture Collection (ATCC).
2. Immunology and Vaccine Development
Heat-inactivated horse serum is crucial in:
- Antibody Production: Used in immunization protocols and hybridoma cell lines.
- Vaccine Formulations: Serves as a stabilizer in viral vaccine production.
- Serological Testing: Employed in diagnostic assays.
For vaccine-related research, check Centers for Disease Control and Prevention (CDC).
3. Biopharmaceutical Research
Horse serum is a fundamental component in pharmaceutical R&D for:
- Protein Expression Studies: Supports recombinant protein production.
- Toxicology Testing: Evaluates drug effects on cultured cells.
- Regenerative Medicine: Used in stem cell differentiation protocols.
For regulatory guidelines, refer to U.S. Food and Drug Administration (FDA).
Safety, Handling, and Storage
To maintain the quality and efficacy of Horse Serum, Heat Inactivated – 100 mL, proper handling is crucial:
- Storage Conditions: Maintain at -20°C or below to preserve bioactivity.
- Thawing Procedures: Slow thawing in a refrigerator to prevent protein precipitation.
- Aseptic Handling: Use sterile techniques to prevent contamination.
For biosafety practices, consult the World Health Organization (WHO).
Regulatory and Ethical Considerations
The collection and processing of horse serum are governed by international safety standards to ensure ethical and high-quality production. Oversight bodies include:
- U.S. Department of Agriculture (USDA) for ethical animal sourcing.
- European Medicines Agency (EMA) for pharmaceutical applications.
- National Institutes of Health (NIH) for biomedical research guidelines.
Conclusion
Horse Serum, Heat Inactivated – 100 mL remains a cornerstone in cell culture, immunology, and pharmaceutical research. With its broad applications in scientific advancements, strict quality control ensures its reliability in laboratory settings.
For continued research and updated guidelines, visit National Center for Biotechnology Information (NCBI) or Blood Safety at the CDC.
By adhering to proper handling protocols and regulatory standards, researchers can maximize the potential of Horse Serum, Heat Inactivated – 100 mL in advancing biomedical science and therapeutic innovations.
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